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gold uptake and tolerance in arabidopsis

Gold uptake and tolerance in Arabidopsis - White Rose ...

21/12/2011  The aims of this study were therefore to characterise gold uptake and nanoparticle formation in the model plant species Arabidopsis thaliana and subsequently investigate the transcriptional response to gold to identify mechanisms of uptake and tolerance. A number of approaches were used to investigate the gold tolerance and uptake by Arabidopsis. Growth studies determined that gold

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British Library EThOS: Gold uptake and tolerance in ...

The aims of this study were therefore to characterise gold uptake and nanoparticle formation in the model plant species Arabidopsis thaliana and subsequently investigate the transcriptional response to gold to identify mechanisms of uptake and tolerance. A number of approaches were used to investigate the gold tolerance and uptake by Arabidopsis. Growth studies determined that gold is toxic to ...

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Gold uptake and tolerance in Arabidopsis - CORE Reader

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Investigating the Toxicity, Uptake, Nanoparticle Formation ...

15/04/2014  To investigate gold uptake by Arabidopsis, plants were grown in liquid culture. After 3 weeks of growth, the plants had produced predominantly shoot and leaf tissues, with approximately 5% dry weight comprising root tissues. After this time, the medium was replaced with water containing a range of gold concentrations (0, 50, 100, 200 and 400 mg/L), in the form of K(AuCl 4)). The

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Nanoparticle Uptake in Plants: Gold Nanomaterial Localized ...

Terrestrial plants can internalize and translocate nanoparticles (NPs). However, direct evidence for the processes driving the NP uptake and distribution in plants is scarce at the cellular level. Here, NP-root interactions were investigated after 10 days of exposure of Arabidopsis thaliana to 10 mgL–1 of negatively or positively charged gold NPs (∼12 nm) in gels. Two complementary ...

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NIP1;2 is a plasma membrane-localized transporter ...

09/05/2017  When heterologously expressed in Arabidopsis, only HmVALT enhances Al tolerance, suggesting that Al may enter the Arabidopsis root cytosol via an unidentified native PM transporter . Thus, in the present study, we investigated the role of the Arabidopsis nodulin 26-like intrinsic protein (NIP) subfamily, the closest homologs of HmPALT1, in Al transport ( SI Appendix , Fig. S1 ).

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Polyploids Exhibit Higher Potassium Uptake and Salinity ...

Uptake and Salinity Tolerance in Arabidopsis Dai-Yin Chao,1 Brian Dilkes, 2Hongbing Luo, Alex Douglas,1 Elena Yakubova,2 Brett Lahner,2 David E. Salt1* Genome duplication (or polyploidization) has occurred throughout plant evolutionary history and is thought to have driven the adaptive radiation of plants. We found that the cytotype of the root, and not the genotype, determined the majority of ...

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Genetic Analysis of Salt Tolerance in Arabidopsis ...

01/07/1998  A large genetic screen for sos (for salt overly sensitive) mutants was performed in an attempt to isolate mutations in any gene with an sos phenotype. Our search yielded 28 new alleles of sos1 , nine mutant alleles of a newly identified locus, SOS2 , and one allele of a third salt tolerance locus, SOS3. The sos2 mutations, which are recessive, were mapped to the lower arm of

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Cadmium uptake, translocation, and tolerance in AHA1OX ...

Cadmium uptake, translocation, and tolerance in AHA1OX Arabidopsis thaliana. Hou L(1), Shi W, Wei W, Shen H. Author information: (1)Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China. [email protected] Information on cadmium (Cd) uptake and transport is essential to understand better the physiology of Cd tolerance in plants. In this study, Cd uptake ...

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Polyploids Exhibit Higher Potassium Uptake and Salinity ...

09/08/2013  Polyploids Exhibit Higher Potassium Uptake and Salinity Tolerance in Arabidopsis. Dai-Yin Chao 1, Brian Dilkes 2, Hongbing Luo 2, Alex Douglas 1, Elena Yakubova 2, Brett Lahner 2, David E. Salt 1, * 1 Institute of Biological and Environmental Sciences, University of Aberdeen, Cruickshank Building, St. Machar Drive, Aberdeen, AB24 3UU, UK. 2 Department of Horticulture and Landscape

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Gold uptake and tolerance in Arabidopsis - CORE

The aims of this study were therefore to characterise gold uptake and nanoparticle formation in the model plant species Arabidopsis thaliana and subsequently investigate the transcriptional response to gold to identify mechanisms of uptake and tolerance. \ud A number of approaches were used to investigate the gold tolerance and uptake by Arabidopsis. Growth studies determined that gold is ...

More

Investigating the Toxicity, Uptake, Nanoparticle Formation ...

15/04/2014  To investigate gold uptake by Arabidopsis, plants were grown in liquid culture. After 3 weeks of growth, the plants had produced predominantly shoot and leaf tissues, with approximately 5% dry weight comprising root tissues. After this time, the medium was replaced with water containing a range of gold concentrations (0, 50, 100, 200 and 400 mg/L), in the form of K(AuCl 4)). The

More

Nanoparticle Uptake in Plants: Gold Nanomaterial

Terrestrial plants can internalize and translocate nanoparticles (NPs). However, direct evidence for the processes driving the NP uptake and distribution in plants is scarce at the cellular level. Here, NP-root interactions were investigated after 10 days of exposure of Arabidopsis thaliana to 10 mgL–1 of negatively or positively charged gold NPs (∼12 nm) in gels. Two complementary ...

More

Arabidopsis and the Genetic Potential for the ...

04/04/2002  Uptake of metal ions. Numerous families of metal ion transporters have been identified in Arabidopsis.These have been extensively reviewed by other authors (Fox and Gueriont, 1998; Clemens, 2001; Maser et al., 2001) and are discussed further below (see Table 1).Some members of these gene families have been identified through the complementation of yeast mutants, while many others have

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NIP1;2 is a plasma membrane-localized transporter ...

09/05/2017  When heterologously expressed in Arabidopsis, only HmVALT enhances Al tolerance, suggesting that Al may enter the Arabidopsis root cytosol via an unidentified native PM transporter . Thus, in the present study, we investigated the role of the Arabidopsis nodulin 26-like intrinsic protein (NIP) subfamily, the closest homologs of HmPALT1, in Al transport ( SI Appendix , Fig. S1 ).

More

Genetic Analysis of Salt Tolerance in Arabidopsis ...

A large genetic screen for sos (for salt overly sensitive) mutants was performed in an attempt to isolate mutations in any gene with an sos phenotype. Our search yielded 28 new alleles of sos1 , nine mutant alleles of a newly identified locus, SOS2 , and one allele of a third salt tolerance locus, SOS3. The sos2 mutations, which are recessive, were mapped to the lower arm of chromosome V, ~2.3 ...

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NIP1;2 is a plasma membrane-localized transporter ...

NIP1;2 is a plasma membrane-localized transporter mediating aluminum uptake, translocation, and tolerance in Arabidopsis Yuqi Wanga, Ruihong Lia,b, Demou Lia,c, Xiaomin Jia a, Dangwei Zhoua,d, Jianyong Lia,e, Sangbom M. Lyi , Siyu Houa,f, Yulan Huanga,g, Leon V. Kochiana,h, and Jiping Liua,1 aRobert W. Holley Center for Agriculture and Health, US Department of Agriculture, Agricultural ...

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PHYSIOLOGICAL AND MOLECULAR DISSECTION OF SALINITY ...

Physiological and Molecular Dissection of Salinity Tolerance in Arabidopsis and Maize and Nitrogen Uptake in Wheat Suman Lamichhane Abstract (Academic) The PROTEOLYSIS 6 (PRT6) branch of the N-end rule pathway is a well-characterized negative regulator of flooding and low oxygen tolerance in plants. This study investigated the role of this pathway in adaptation to salinity stress in ...

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WRKY6 Transcription Factor Restricts Arsenate Uptake

The pht1;1 pht1;4 double mutant has a notable As(V) tolerance phenotype, indicating that these two members of the Pi transporter family contribute to As(V) uptake in Arabidopsis (Shin et al., 2004). Some species restrict phosphate uptake as an adaptive response in As(V) tolerance ( Meharg and Macnair, 1990 ; Murota et al., 2012 ).

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Cellular mechanisms for heavy metal detoxification and ...

01/01/2002  A new vertical mesh transfer technique for metal‐tolerance studies in Arabidopsis. Plant Physiology. 108, 29 –38. Murphy A, Taiz L. 1995. b. Comparison of metallothionein gene expression and nonprotein thiols in ten Arabidopsis ecotypes. Plant Physiology. 109, 945 –954. Neumann D, Lichtenberger O, Günther D, Tschiersch K, Nover L. 1994. Heat‐shock proteins induce heavy‐metal ...

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Gold uptake and tolerance in Arabidopsis - CORE

The aims of this study were therefore to characterise gold uptake and nanoparticle formation in the model plant species Arabidopsis thaliana and subsequently investigate the transcriptional response to gold to identify mechanisms of uptake and tolerance. \ud A number of approaches were used to investigate the gold tolerance and uptake by Arabidopsis. Growth studies determined that gold is ...

More

gold uptake and tolerance in arabidopsis

gold uptake and tolerance in arabidopsis. Transcriptome profiling of genes and pathways associated ... To identify genes associated with As toxicity and tolerance in Arabidopsis, we used large-scale expression profiling. The As exposure data (Figure 1) helped determine a suitable As concentration for microarray analysis. Treatment of Col-0 and Ws-2 with As at 200 and 100 μM, respectively, had ...

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Investigating the Toxicity, Uptake, Nanoparticle Formation ...

15/04/2014  To investigate gold uptake by Arabidopsis, plants were grown in liquid culture. After 3 weeks of growth, the plants had produced predominantly shoot and leaf tissues, with approximately 5% dry weight comprising root tissues. After this time, the medium was replaced with water containing a range of gold concentrations (0, 50, 100, 200 and 400 mg/L), in the form of K(AuCl 4)). The

More

Nanoparticle Uptake in Plants: Gold Nanomaterial

Terrestrial plants can internalize and translocate nanoparticles (NPs). However, direct evidence for the processes driving the NP uptake and distribution in plants is scarce at the cellular level. Here, NP-root interactions were investigated after 10 days of exposure of Arabidopsis thaliana to 10 mgL–1 of negatively or positively charged gold NPs (∼12 nm) in gels. Two complementary ...

More

Arabidopsis and the Genetic Potential for the ...

04/04/2002  Uptake of metal ions. Numerous families of metal ion transporters have been identified in Arabidopsis.These have been extensively reviewed by other authors (Fox and Gueriont, 1998; Clemens, 2001; Maser et al., 2001) and are discussed further below (see Table 1).Some members of these gene families have been identified through the complementation of yeast mutants, while many others have

More

Genetic Analysis of Salt Tolerance in Arabidopsis ...

A large genetic screen for sos (for salt overly sensitive) mutants was performed in an attempt to isolate mutations in any gene with an sos phenotype. Our search yielded 28 new alleles of sos1 , nine mutant alleles of a newly identified locus, SOS2 , and one allele of a third salt tolerance locus, SOS3. The sos2 mutations, which are recessive, were mapped to the lower arm of chromosome V, ~2.3 ...

More

PHYSIOLOGICAL AND MOLECULAR DISSECTION OF SALINITY ...

Physiological and Molecular Dissection of Salinity Tolerance in Arabidopsis and Maize and Nitrogen Uptake in Wheat Suman Lamichhane Abstract (Academic) The PROTEOLYSIS 6 (PRT6) branch of the N-end rule pathway is a well-characterized negative regulator of flooding and low oxygen tolerance in plants. This study investigated the role of this pathway in adaptation to salinity stress in ...

More

WRKY6 Transcription Factor Restricts Arsenate Uptake

The pht1;1 pht1;4 double mutant has a notable As(V) tolerance phenotype, indicating that these two members of the Pi transporter family contribute to As(V) uptake in Arabidopsis (Shin et al., 2004). Some species restrict phosphate uptake as an adaptive response in As(V) tolerance ( Meharg and Macnair, 1990 ; Murota et al., 2012 ).

More

Cellular mechanisms for heavy metal detoxification and ...

01/01/2002  A new vertical mesh transfer technique for metal‐tolerance studies in Arabidopsis. Plant Physiology. 108, 29 –38. Murphy A, Taiz L. 1995. b. Comparison of metallothionein gene expression and nonprotein thiols in ten Arabidopsis ecotypes. Plant Physiology. 109, 945 –954. Neumann D, Lichtenberger O, Günther D, Tschiersch K, Nover L. 1994. Heat‐shock proteins induce heavy‐metal ...

More

Brassica oleracea MATE Encodes a Citrate Transporter and ...

Al tolerance was determined by measuring relative root elongation according to Liang et al. (2013). In brief, seeds of wild-type Arabidopsis and two transgenic lines were surface sterilized, stratified at 4°C for 3 days, and then germinated for 4 days on MS medium.

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